发明名称 |
Novel dna cloning method |
摘要 |
The invention refers to a novel method for cloning DNA molecules using a homologous recombination mechanism between at least two DNA molecules comprising: a) providing a host cell capable of performing homologous recombination, b) contacting in said host cell a first DNA molecule which is capable of being replicated in said host cell with a second DNA molecule comprising at least two regions of sequence homology to regions on the first DNA molecule, under conditions which favour homologous recombination between said first and second DNA molecules and c) selecting a host cell in which homologous recombination between said first and second DNA molecules has occurred. |
申请公布号 |
AU1877199(A) |
申请公布日期 |
1999.06.28 |
申请号 |
AU19990018771 |
申请日期 |
1998.12.07 |
申请人 |
EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL) |
发明人 |
FRANCIS STEWART;YOUMING ZHANG;FRANK BUCHHOLZ |
分类号 |
C12N15/09;C12N1/21;C12N15/10;C12N15/90;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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