| 摘要 |
A chimeric DNA sequence comprising the maize histone H3C4 promoter associated with the first intron of the rice actin gene, is new. A DNA sequence comprising a 5' regulatory element for the expression of a heterologous gene in a monocotyledonous vegetable plant cell is characterized in that the DNA comprises: (a) a first DNA sequence that is a functional fragment of the maize H3C4 promoter sequence; and (b) a second DNA sequence that is a functional fragment of the first intron of the rice actin gene. Independent claims are also included for the following: (1) a chimeric gene comprising a coding sequence as well as the heterologous regulatory elements in the 5' and 3' positions for functioning in monocotyledonous vegetable plant cells or monocotyledonous plants, characterized in that the 5' regulatory sequence comprises the DNA sequence as above; (2) a DNA sequence encoding an OTP/CP4 (optimized transit peptide-calcium-dependent protein kinase) fusion protein; (3) an OTP/CP4 fusion protein; (4) a vector for cloning or expression, for transformation of a vegetable or plant cell, characterized in that it comprises a chimeric gene as above with at least one origin of replication; (5) a method for transformation of vegetable cells by integrating a chimeric gene as above; (6) a vegetable cell characterized in that it contains at least one chimeric gene as above; (7) a transformed plant characterized in that it comprises cells as in (6); and (8) grain of a transformed plant as above.
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