MASS SPECTROMETRIC METHODS FOR SEQUENCING NUCLEIC ACIDS
摘要
A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods include use the RNA polymerase is to generate a set of nested RNA transcripts, which are analyzed mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.
申请公布号
CA2314906(A1)
申请公布日期
1999.06.24
申请号
CA19982314906
申请日期
1998.12.15
申请人
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY;SEQUENOM, INC.
发明人
O'DONNELL, MARYANNE J.;KANG, CHANGWON;KWON, YOUNG-SOO;CANTOR, CHARLES;LOUGH, DAVID M.;LITTLE, DANIEL P.;KOSTER, HUBERT;KIM, YOUNG TAE;XIANG, GUOBING