| 摘要 |
<p>A method for determining a base sequence of a nucleotide strand in a sample utilizes a probe including fluorescent metal-ligand complex coupled to a first oligonucleotide having a sequence complementary to first fragment of the strand. The first mixture is exposed to an exciting amount of radiation, and the fluorescence of the metal-ligand complex is detected. The first base sequence of the first fragment is identified based on fluorescence of the metal-ligand complex. A second probe differing from the first by at least one base is provided. A second base of the second fragment is identified based on the fluorescence of the metal-ligand complex of the second probe. The second base sequence is compared to the first base sequence to identify a difference between the first and second sequences to determine a base sequence of the nucleotide strand.</p> |
