| 摘要 |
<p>The present invention relates to an improved method for the identification and optionally the characterisation of interacting molecules designed to perform two-hybrid screening in a high throughput fashion. The method of the invention relies on a combination of automated steps used to generate and detect clones that express interacting molecules, and to separate positive from false positive clones. The present invention provides for high-throughput interaction screens for the reliable identification of interacting molecules, which in turn can lead to the identification of substances inhibiting said interactions. Such inhibitors can find their use in the formulation of a pharmaceutical composition.</p> |
