| 摘要 |
Detecting or determining a solid-phase associated factor (F), which is associated with the solid phase a number of times within the sample and comprises contacting the sample with a first and second stabile complex (C1 and C2) comprising at least one ligand (L) with a binding affinity for F, a sender (S) and a receiver (E), to form complexes. Detecting or determining a solid-phase associated factor (F), which is associated with the solid phase a number of times within the sample and comprises contacting the sample with a first and second stabile complex (C1 and C2) comprising at least one ligand (L) with a binding affinity for F, a sender (S) and a receiver (E), to form the complexes F-L-S and F-L-E. When E and S are in close vicinity a signal is generated which can be measured. An Independent claim is also included for a method for simultaneously detecting and determining at least two solid phase associated factors Fx and Fy, which are both associated with the same solid phase in a sample, comprising contacting the sample with C1, where L (Lx) is specific for Fx, to form a complex Fx-Lx-S, and C2, where L (Ly) is specific for Fy, to form a complex Fy-Ly-E. When E and S are in close vicinity a signal is generated which can be measured.
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