发明名称 METHOD FOR PRODUCING TRANSGENIC PLANTS WITH MODIFIED 5-AMINOLEVULINIC ACID BIOSYNTHESIS, METHOD FOR IDENTIFYING 5-AMINOLEVULINIC ACID SYNTHESIS EFFECTORS
摘要 The invention relates to a method for producing transgenic plants by means of modified 5-aminolevulinic acid biosynthesis, transgenic plant cells, transgenic plant parts, transgenic seeds, and transgenic reproduction material. The invention is characterised in that one or several nucleic acid molecules coding for a protein with a 5-aminolevulinic acid synthase function (ALAS), selected from a group of feedback regulated ALAS, animal ALAS and bacterial ALAS, an active fragment thereof or an antisense or complementary sequence thereof, are integrated into the plant genome in a stable form. Effectors of natural 5-aminolevulinic acid biosynthesis in plants are found using a method for determining effector efficacy of a test substance in relation to a GSAAT, by: a) determining the enzymatic activity of the GSAAT in the absence of a test substance b) determining the enzymatic activity of the GSAAT in the presence of a test substance; and c) by comparing the enzymatic activities determined under a) and b).
申请公布号 CA2313706(A1) 申请公布日期 1999.06.17
申请号 CA19982313706 申请日期 1998.12.10
申请人 AVENTIS CROPSCIENCE GMBH 发明人 SCHMIDT, FRANK;DONN, GUNTER
分类号 A01H5/00;C12N5/10;C12N9/10;C12N9/90;C12N15/09;C12N15/31;C12N15/60;C12N15/82;G01N33/15;G01N33/50;(IPC1-7):C12N15/82 主分类号 A01H5/00
代理机构 代理人
主权项
地址