发明名称 |
Coupled amplification and ligation method |
摘要 |
A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel. The method involves simultaneously incubating a sample containing one or more target polynucleotides with PCR primers and OLA probes in a single reaction mixture. The presence of variant polynucleotide sequences in the sample is determined by detecting and identifying the products of the OLA reaction.
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申请公布号 |
US5912148(A) |
申请公布日期 |
1999.06.15 |
申请号 |
US19960975902 |
申请日期 |
1996.09.19 |
申请人 |
PERKIN-ELMER CORPORATION APPLIED BIOSYSTEMS |
发明人 |
EGGERDING, FAYE |
分类号 |
C12N15/09;C07H21/02;C07H21/04;C12P19/34;C12Q1/68;(IPC1-7):C12P19/34;C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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