PURIFIED TRANSCRIPTION FACTOR CDF-1 AND ITS USE

摘要

The invention provides a method for obtaining the transcription factor CDF-1 in purified form, as well as assays which related to the modulation of CDF-1 repression of transcription induced by viral oncogenes, particularly HPV E7. Provided is an assay for a modulator of a viral replication, wherein said replication is facilitated by suppression of cdc25 promoter repression by CDF-1 due to the action of a viral protein, which assay comprises: a) bringing said viral protein into contact with one or both of: (i) a CDF-1 protein; and (ii) a DNA sequence comprising a CDF-1 binding site, in the presence of a putative modulator under conditions wherein in the absence of modulator the viral protein is able to induce inhibition of CDF-1 binding to DNA; and b) measuring the degree of modulation of binding of CDF-1 to DNA.