发明名称 |
PURIFIED TRANSCRIPTION FACTOR CDF-1 AND ITS USE |
摘要 |
The invention provides a method for obtaining the transcription factor CDF-1 in purified form, as well as assays which related to the modulation of CDF-1 repression of transcription induced by viral oncogenes, particularly HPV E7. Provided is an assay for a modulator of a viral replication, wherein said replication is facilitated by suppression of cdc25 promoter repression by CDF-1 due to the action of a viral protein, which assay comprises: a) bringing said viral protein into contact with one or both of: (i) a CDF-1 protein; and (ii) a DNA sequence comprising a CDF-1 binding site, in the presence of a putative modulator under conditions wherein in the absence of modulator the viral protein is able to induce inhibition of CDF-1 binding to DNA; and b) measuring the degree of modulation of binding of CDF-1 to DNA.
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申请公布号 |
WO9927092(A1) |
申请公布日期 |
1999.06.03 |
申请号 |
WO1998GB03486 |
申请日期 |
1998.11.23 |
申请人 |
PROLIFIX LIMITED;MUELLER, ROLF;LIU, NINGSHU |
发明人 |
MUELLER, ROLF;LIU, NINGSHU |
分类号 |
C07K14/47;C12N15/12;(IPC1-7):C12N15/12;C12Q1/68;G01N33/68;C07K16/18 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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