| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new model animal comprising a mammalian or the like obtained by generating an individual from a totipotent cell containing human presenilin 2 gene or the like, having the gene in the chromosomes of body cells, and useful for analyzing the states of Alzheimer's diseases, developing medicines therefor, and the like. SOLUTION: This model animal for Alzheimer's diseases comprises a mammalian obtained by generating an individual from a totipotent cell containing human presenilin 2 gene or its variant transduced thereinto or the progeny of the mammalian, wherein the transduced gene is held in the chromosomes of body cells. The model animal is used for analyzing the states of Alzheimer's diseases, developing medicines for treating the diseases, and the like. The model animal for the Alzheimer's diseases is obtained by extracting all RNAs from the brain of a healthy old person, preparing cDNAs from the all RNAs, obtaining a wild type presenilin 2 cDNA by a PCR amplification method or preparing a variant cDNA in which a mutation is partially introduced by a PCR mutation-inducing method, inserting the product into a vector, injecting the vector into a mouse embryo, injecting the embryo into a provisional parent oviduct, allowing to generate an individual, and subsequently making to bear the individual. |
