发明名称 |
RECOMBINANT LIBRARY SCREENING METHODS |
摘要 |
<p>Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vector, or into a sequence encoding a protein which may be linked by means of a ligand to a phage coat protein. By employing affinity purification techniques the phage particles containing sequences encoding the desired protein may be selected and the desired nucleotide sequences obtained therefrom. Thus, for example, novel proteins such as monoclonal antibodies may be produced and conventional hybridoma technology avoided.</p> |
申请公布号 |
GR3029421(T3) |
申请公布日期 |
1999.05.28 |
申请号 |
GR19990400504T |
申请日期 |
1999.02.17 |
申请人 |
AFFYMAX TECHNOLOGIES N.V. |
发明人 |
DOWER, WILLIAM, J.;CWIRLA, STEVEN, E. |
分类号 |
C07K14/00;C07K16/00;C07K19/00;C12N1/21;C12N7/00;C12N15/09;C12N15/10;C12P21/02;C12P21/08;C12Q1/68;C12R1/92;C40B40/02;G01N33/53;G01N33/531;(IPC1-7):C12Q1/70;G01N33/68;C12N15/00;C12N1/20;G01N33/566 |
主分类号 |
C07K14/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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