Method for the isolation and purification of nucleotid-activated sugars from biological sources

摘要

Isolation and purificn. of sugar nucleotides (I) from biological sources (i.e. an enzymatic reaction mixt. or a cell extract, where necessary, after removal of insol. cellular components) comprises removal of dissolved proteins by pptn. with alcohol, evapn. to dryness and chromatography of the (I)-contg. residue on silica gel. The new feature is that the residue is taken up in a 1:1-1:10 (by vol.) mixt. of lower alcohol and 0.5-1 M aq. NH4 salt soln., blended with dry silica gel to form a mass with honey-like consistency and applying this to a column of commercial silica gel of appropriate grain size. The column is then eluted, under pressure, with the specified solvent mixt.