A METHOD FOR DIRECTIONAL STABLE TRANSFORMATION OF EUKARYOTIC CELLS
摘要
<p>The present invention is drawn to compositions and methods for introducing nucleotide sequences at preferred genomic target sites in a eukaryotic genome. The compositions comprise transfer cassettes which are flanked by nonhomologous recombination sites. The method involves transforming eukaryotic cells containing target sites utilizing non-integrating transformation methods. The method results in efficient integration of nucleotides into predetermined genetic locations and eliminates random DNA integration.</p>
申请公布号
WO9925854(A1)
申请公布日期
1999.05.27
申请号
WO1998US24609
申请日期
1998.11.17
申请人
PIONEER HI-BRED INTERNATIONAL, INC.;GORDON-KAMM, WILLIAM, J.;LYZNIK, ALEXANDER, LESZEK;BASZCZYNSKI, CHRISTOPHER, L.
发明人
GORDON-KAMM, WILLIAM, J.;LYZNIK, ALEXANDER, LESZEK;BASZCZYNSKI, CHRISTOPHER, L.