发明名称 |
CULTURE VIBRIO CHOLERAE, METHOD OF DELETION MUTANTS PRODUCING |
摘要 |
FIELD: microbiology, molecular genetics. SUBSTANCE: method involves isolation of vaccine strain of Vibrio cholerae which is alternated specifically by technique of recombinant DNA in order to eliminate its virulence without damage of other components that are necessary for immunity. This attenuation is carried out using digestion of plasmids with restriction endonucleases. These plasmids carry the corresponding DNA nucleotide sequences of V. cholerae. Strains without genes encoding toxin or its fragments are obtained by transfer of ligated gene of digested plasmids and the following selection procedures of recombinants with virulent hosts of V. cholerae in vivo. Vaccine prepared from this strain causes the stable immunity for cholera control. EFFECT: improved method of mutants producing. 7 cl, 1 tbl, 40 dwg, 11 ex
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申请公布号 |
RU2130970(C1) |
申请公布日期 |
1999.05.27 |
申请号 |
SU19915011181 |
申请日期 |
1991.06.05 |
申请人 |
JUNIVERSITI OF MEHRILEHND EHT BALTIMOR |
发明人 |
DZHEJMS B.KAPER;BERNADETT BADRI-MARELLI;ALESSIO FASANO |
分类号 |
A61K39/106;C07K14/28;C12N1/19;C12N1/21;C12N15/09;C12N15/30;C12R1/63;(IPC1-7):C12N15/30 |
主分类号 |
A61K39/106 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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