发明名称 |
VARIANT loxP SEQUENCES AND APPLICATION OF THE SAME |
摘要 |
Variant loxP sequences having the characteristics (a) to (c) as specified below whereby gene insertion or gene substitution can be effected at a high efficiency in higher eucaryotic cells such as animal cells: (a) having a sequence derived from the following wild type loxP sequence originating in a coliphage P1 by substitution of at least one of the bases at the 2-, 3-, 4- and 5-positions (i.e., T, G, T and A, respectively) and at least one of the bases at the 6- and 7-positions (i.e., T and G, respectively) in the central eight bases (the spacer region) each by a different base optionally with substitution of base(s) in the regions other than the spacer region by arbitrary base(s): 12345678 5'-ATAACTTCGTATA <u>ATGTATGC</u> TATACGAAGTTAT-3' SPACER REGION; (b) not undergoing specific DNA recombination with the wild type loxP sequence even in the presence of a recombinase Cre; and (c) undergoing specific DNA recombination with another variant loxP sequence having the same sequence in the presence of the recombinase Cre.
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申请公布号 |
WO9925851(A1) |
申请公布日期 |
1999.05.27 |
申请号 |
WO1998JP05094 |
申请日期 |
1998.11.12 |
申请人 |
SUMITOMO PHARMACEUTICALS COMPANY, LIMITED;SAITO, IZUMU;TANAKA, KEIJI |
发明人 |
SAITO, IZUMU;TANAKA, KEIJI |
分类号 |
A01K67/027;A01K67/033;A61K48/00;C12N5/10;C12N7/00;C12N15/00;C12N15/09;C12N15/861;C12N15/90;C12R1/92;(IPC1-7):C12N15/66;C12N15/79;C12N5/06 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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