Method for storing red cells using reduced citrate anticoagulant and a solution containing sodium, citrate, phosphate, adenine and mannitol

摘要

The present invention provides an improved aqueous solution for suspending and storing red blood cells. The solution includes sodium citrate, a combination of sodium biphosphate and sodium phosphate dibasic, adenine, and mannitol. The solution is formulated at a low osmolarity and has a physiological pH of approximately 7.4. The solution simultaneously maintains stored red cells ATP and 2,3-BPG. Also disclosed is a method for storing red blood cells comprising adding an anticoagulant with a reduced citrate concentration compared to a CPD solution comprising 26.3 g/l, sodium citrate and 3.28 g/l citric acid hydrous to whole blood, separating the red cells and adding a storage solution comprising sodium citrate, sodium diphosphate, sodium phosphate dibasic, adenine and mannitol.