| 摘要 |
PROBLEM TO BE SOLVED: To efficiently obtain the subject transferase useful for diagnosis or the like in high purity and yield, by culturing a specific transformant to produce β-1,4-galactose transferase or the like derived from human and treating the resultant enzyme. SOLUTION: Escherichia coli is transformed with an expression vector such as pMsGT in which a gene encoding 1,4-galactose transferase derived from human, e.g. a gene isolated from cDNA derived from human placenta and encoding an amino acid sequence up to 66-386th amino acid sequence represented by the formula and a gene encoding a maltose-bound protein, e.g. a gene derived from pMAL-p2 or pMAL-c2 are integrated and the resultant transformant is cultured. Thereby, a fused protein comprising β-1,4-galactose transferase derived from human and maltose-bound protein is produced and the fused protein is purified by affinity chromatography and decomposed by a factor Xa to provide the objective β-1,4-galactose transferase derived from human. |
