| 摘要 |
PROBLEM TO BE SOLVED: To obtain a composition useful in e.g. immunoassay and containing colloid particles with biomolecule adsorbed onto the surface, by adding a surfactant to a biomolecule solution when this solution is to be loaded on the colloid particles. SOLUTION: This composition is obtained by adding a surfactant (e.g. ethoxylate surfactant) so as to be 0.0001-1 mM in concentration to colloid particles before loading the colloid particles with biomolecule such as protein, glycoprotein, peptide, nucleic acid, peptide nucleic acid, saccharide, antigen and/or hapten, and/or by adding the surfactant to a solution containing the biomolecule before and/or during loading the colloid particles with the solution containing the biomolecule. This composition contains a conjugate consisting of the colloid particles such as noble metal particles with the biomolecule adsorbed onto the surface, also containing the surfactant, and being useful as e.g. a detective reagent using immunological detection method. |
