METHODS FOR IDENTIFYING BIOLOGICAL MACROMOLECULE INTERACTIONS WITH COMPOUNDS

摘要

Methods for determining whether a biological macromolecule binds to a compound. The methods comprise the steps of: a) obtaining an FT-IR spectrum of a solution comprising a biological macromolecule and obtaining an FT-IR spectrum of a solution comprising a compound; b) obtaining an FT-IR spectrum of a solution comprising a mixture of the biological macromolecule and the compound; c) subtracting the FT-IR spectrum of the individual biological macromolecule and compound from the FT-IR spectrum of the mixture to determine a subtracted index; and d) identifying a compound that binds to the biological macromolecule when the subtracted index of step c) is not equal to about 0.