Combinatorial strategies for polymer synthesis

摘要

There is disclosed a method of forming oligonucleotides or peptides having diverse sequences on a single substrate, said substrate comprising a surface with a plurality of selected regions with acid-labile protecting groups protecting activated sites capable of covalently binding nucleic acids or amino acids, said method comprising the steps of: (a) providing on said surface a photoresist wherein said photoresist is an acid generating polymer; (b) selectivity irradiating a portion of said plurality of selected regions whereby acid is generated thereby removing said acid-labile protecting groups to provide said activated sites in said selectivity irradiated portion; (c) removing said photoresist in said selectivity irradiated portion of said plurality of selected regions; (d) contacting and covalently binding selected nucleic acids or amino acids to said activated sites in said selectively irradiated portion; (e) sequentially contacting and covalently bonding one or more selected nucleic acids or amino acids to the substrate of step d) to form said oligonucleotides or peptides having diverse sequences at the selectively irradiated portions. Also disclosed is a method of forming an array of diverse molecules on a surface of a substrate.