A gas chromatographic method utilizes columns having either ascending or descending temperature profiles to effect separation even under column overloading conditions. A fixed ascending temperature profile column allows for good separation of components of a mixture under conditions of column overloading. A mixture to be separated is chromatographed on at least one such column prior to chromatography on a column maintained with a descending temperature profile. This latter column has surprisingly been found to greatly reduce tailing of less volatile species. The invention also includes a variable temperature descending column chromatography to provide an initial ordering of a large quantity of components prior to chomatography on the fixed ascending temperature profile column, or on the serial fixed ascending and descending temperature profile pair of columns.
申请公布号
US5904749(A)
申请公布日期
1999.05.18
申请号
US19940186900
申请日期
1994.01.27
申请人
CHEN, CHRISTOPHER HUK-SHI;CHANG, SHINN-DER;WENG, CHENGWEN;TANG, YUANMING;HOLZHUETER, INGO BERND;HAWTHORNE, SAMUEL HUGH
发明人
CHEN, CHRISTOPHER HUK-SHI;CHANG, SHINN-DER;WENG, CHENGWEN;TANG, YUANMING;HOLZHUETER, INGO BERND;HAWTHORNE, SAMUEL HUGH