VERFAHREN ZUR TRENNUNG VON HIGH DENSITY LIPOPROTEIN AUS BLUTPROBEN

摘要

A method (I) of selectively separating high density lipoprotein (HDL) from a blood sample contg. HDL, low DL and very low DL and chylomicrons comprises contacting the sample with finely divided particulate, porous silica or silicate having a particle size of LU-1000 microns in the longest dimension and surface pores of 80-1000 Angstroms in size as absorbent particles to form a complex between the silica or silicate and the HDL and separating the complex from the blood sample by solid/liquid separatory techniques, to provide a blood sample substantially free of HDL. In (I) the absorbent particles are pref. 2-500 microns in their longest dimension and the pores are 300-500 Angstrom in size. The absorbent material is pref. silica, silica gel or controlled pore glass. The second reagent system comprises a polyanion, e.g. heparin or dextran sulphate and a cation is Mn2+ or Mg2+.