CLOSED SYSTEM FOR PROCESSING PRIMARY TISSUE

摘要

The present invention relates to methods and kits for tumor processing in a closed environment, using blunt dissection by solid particles. The tissue dissociation time is significantly less than that needed for conventional methods (minutes versus hours). The methods and kit of the invention allow greater surface areas of tumor to be dissociated at any one time due to the increased bead/tumor contact surface area. Primary tumors were reproducibly dissociated into viable heterogeneous single cell suspensions using the methods and kit of the invention, while retaining cell function.