发明名称 |
METHOD FOR COLLECTING HEMOLYMPH OF INSECTS |
摘要 |
The present invention herein provides a method for collecting insect hemolymph which permits the collection of hemolymph, at a time, from a vast number of insect bodies, which does not cause any scattering of the hemolymph during collection thereof, which may widely be used, which permits the collection of the hemolymph free of unnecessary tissues or the like and which can inhibit the melanization of the collected hemolymph. The insect hemolymph can be collected by freezing anesthetized lepidopterous insects, piercing the epidermis of the frozen insects without damaging the alimentary canals thereof and then thawing them in a buffer solution containing a melanization-inhibitory agent to thus discharge the hemolymph of the insects into the buffer solution through the holes while making use of the self-contraction phenomenon caused during the thawing process. |
申请公布号 |
CA2210262(C) |
申请公布日期 |
1999.05.11 |
申请号 |
CA19972210262 |
申请日期 |
1997.04.28 |
申请人 |
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NATIONAL INSTITUTE OF SERICU LTURAL AND ENTOMOLOGICAL SCIENCE MINISTRY OF AGRICULTURE, FORESTRY AND F ISHERIES |
发明人 |
MIYAZAWA, MITSUHIRO;ARAKAWA, TORU |
分类号 |
A01K67/033;C07K14/435 |
主分类号 |
A01K67/033 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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