Process for the production of D- alpha -amino acids by hydrolysis of the corresponding N-carbamyl derivative

摘要

PCT No. PCT/JP93/01408 Sec. 371 Date Jun. 6, 1994 Sec. 102(e) Date Jun. 6, 1994 PCT Filed Oct. 1, 1993 PCT Pub. No. WO94/08030 PCT Pub. Date Apr. 14, 1994In a process for the production of a D- alpha -amino acid, in which an N-carbamyl-D- alpha -amino acid corresponding to the general formula: wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D- alpha -amino acid corresponding to the general formula: wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D- alpha -amino acid to the D- alpha -amino acid. The conversion of the N-carbamyl-D- alpha -amino acids to the D- alpha -amino acids is carried out in a neutral to alkaline pH range.