摘要 |
The invention relates to a method for the assay of TNF- alpha induced airways contraction; which comprises treating isolated airways musculature with a cell permeabilisation agent in a calcium controlled buffer solution to control calcium transport through a cell wall thereby to control contraction of the musculature in a concentration dependent fashion to give a first contraction value; relaxing the treated musculature in a calcium controlled buffer solution and subsequently treating the relaxed musculature with TNF- alpha and exposing to a calcium buffer solution to obtain a second contraction value; and comparing said contraction values. |