Human T-cellreceptors, their uses in diagnosis and therapy of diabetes mellitus.

摘要

New nucleic acid (NA) encoding a section of the CDR3-region of a human T-cell receptor chain has one of the sequences (I)-(VI) or a degenerate sequence having at least 80% homology to them: CTAGAGAACACAGGC (I) CTGAGTGAGG CCCCGAATTA TGGTGGTGCT ACAAAC (II) GTGACCACTC AGTTTTCTGG TGGCTAC AAT (III) AGTAGTGACA GGTTAGGCAA TCAGCCC (IV) AGCCAAGAT CGACTGAGGG GTGTCGCAGAT ACG (V) AGCCAAGAGG CCGACATT (VI) The sequence pref. encodes the entire CDR3-region of an alpha -chain of the human T-cell receptor. Also claimed are : (1) vector contg. at least one copy of the NA; (2) cells contg. the NA or vector, (3) polypeptides encoded by the NA, (4) antibodies to the polypeptides of (3), (5) a polypeptide (A) having T-cell characteristics and comprising a two subunits, one from an alpha -chain and the other from a beta -chain of a human T-cell receptor (or derivs.); (6) complex comprising (A) and a peptide (B) presenting HLA mol. of the class DQw1; and (7) T-cells including (A).