Method for the detection of ras oncogenes, in particular the K-ras ancogene

摘要

PCT No. PCT/GB95/02644 Sec. 371 Date Aug. 22, 1997 Sec. 102(e) Date Aug. 22, 1997 PCT Filed Nov. 10, 1995 PCT Pub. No. WO96/15262 PCT Pub. Date May 23, 1996The invention relates to an oligonucleotide primer sequence for use in in vitro amplification, characterised in that said primer sequence is capable of creating a BstX I restriction site overlapping codon (12) and/or an Xcm I restriction site overlapping codon (13) or a Bce 83I restriction site overlapping codon (61) of the wild-type K-ras oncogene, methods of using said primer sequences for detecting activating mutations in codons (12 and/or 13 and/or 61) of the K-ras oncogene and kits for performing the methods.