FLORAL ORGAN-SPECIFIC PROMOTER SEQUENCES

摘要

This invention provides a flower organ-specific promoter enabling genetic engineering manipulations of pistil or lodicule. It further provides a novel chitinase expressed specifically in flower organ and its gene. The flower organ-specific promoter of the present invention is contained in the sequence consisting of nucleotides of positions from 1 to 1234 in the nucleotide sequence represented by SEQ ID NO:2 in Sequence Listing. The promoter of the present invention includes sequences derived from the above sequence by deletion, substitution, insertion or addition of one or more nucleotides and having a flower organ-specific promoter activity and also those obtainable by using the nucleotide sequence represented by SEQ ID NO:1 in Sequence Listing as a probe and having a flower organ-specific promoter activity. The flower organ-specific chitinase gene of the present invention is one consisting of the nucleotide sequence of positions from 1 to 1097 in the nucleotide sequence represented by SEQ ID NO:1 in Sequence Listing. Moreover, it involves a part of this sequence and DNA fragments having a sequence derived from said sequences by deletion, substitution, insertion or addition of one or more nucleotides and encoding a protein having a biological activity equivalent to that of the protein encoded by the DNA consisting of the above nucleotide sequence. The flower organ-specific chitinase of the present invention consists of the amino acid sequence represented by SEQ ID NO:3 in Sequence Listing. Moreover, it includes a part of this amino acid sequence or amino acid sequences having a sequence derived from these amino acids by deletion, substitution, insertion or addition of one or more amino acids and constituting a protein having a biological activity equivalent to that of the protein consisting of the above amino acid sequence.