METHODS FOR IDENTIFYING NUCLEIC ACID SEQUENCES AND (POLY)PEPTIDES WHICH INCREASE THE EXPRESSION YIELDS OF FUNCTIONAL PERIPLASMIC PROTEINS

摘要

The present invention relates to a method for obtaining nucleic acid sequence encoding (poly)peptides which increase the expression yields of periplasmic proteins in functional form upon co-expression of said (poly)peptides and said periplasmic proteins. The invention also provides a method for the identification of said (poly)peptides. Furthermore, the present invention relates to a method for increasing the expression yields of periplasmic proteins in functional form by co-expressing (poly)peptides, for example Skp, FkpA, or a homolog of Skp or FkpA, in bacteria.