Method for non-radioactive gel shift assays

摘要

A method for a non-radioactive electrophoretic mobility shift assay performed with non-radioactive labeled dsDNA, non-radioactive labeled ssDNA or non-radioactive labeled RNA probes interacting with a nucleic acid binding protein in forming a complex, electrophoresing the mixture containing the complex, transferring the complex to a membrane, and detecting the complex transferred to the membrane by detecting the non-radioactive label in the complex.