Hybrid receptors nucleic acid encoding them their preparation and their use in determination of ligands and their antagonists and agonists

摘要

Hybrid receptors, produced by recombinant DNA technology, comprise (a) the ligand binding domain of a predetermined receptor and (b) a heterologous reporter polypeptide. The hybrid receptors are useful for convenient and large scale assay of biologically active ligands or their antagonists or agonists. (a) may be the extracellular domain of the receptor, or a cytoplasmic domain of a receptor or oncogene. (b) may be an enzyme. A transmembrane domain may be interposed between (a) and (b).