发明名称 |
NOVEL MODIFIED MSP-1 NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING MRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS |
摘要 |
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of malarial surface protein MSP-1 which is known to be difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred protein candidates for expression using the recombinant techniques of the invention are MSP-1 proteins expressed from DNA coding sequences comprising reduced overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the native MSP-1 gene.
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申请公布号 |
CA2306796(A1) |
申请公布日期 |
1999.04.29 |
申请号 |
CA19982306796 |
申请日期 |
1998.10.28 |
申请人 |
GENZYME TRANSGENICS CORPORATION |
发明人 |
MEADE, HARRY;CHEN, LI HOW |
分类号 |
A01K67/027;A61K35/76;A61K39/00;A61K39/012;A61K39/12;A61K48/00;C07H21/04;C07K14/445;C07K14/47;C12N1/21;C12N5/10;C12N15/09;C12N15/30;C12N15/67;C12N15/74;C12N15/85;C12P19/34;(IPC1-7):C12N15/67 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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