发明名称 |
NOVEL MODIFIED NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS |
摘要 |
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of proteins which are known to be, or are likely to be, difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred "difficult" protein candidates for expression using the recombinant techniques of the invention are those proteins derived from heterologous cells preferably those of lower organisms such as parasites, bacteria, and virus, having DNA coding sequences comprising high overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the recombinant expression system to be used. |
申请公布号 |
WO9920766(A2) |
申请公布日期 |
1999.04.29 |
申请号 |
WO1998US22225 |
申请日期 |
1998.10.20 |
申请人 |
GENZYME TRANSGENICS CORPORATION |
发明人 |
CHEN, LI, HOW;MEADE, HARRY |
分类号 |
A01K67/027;A61K35/76;A61K39/00;A61K39/012;A61K39/12;A61K48/00;C07H21/04;C07K14/445;C07K14/47;C12N1/21;C12N5/10;C12N15/09;C12N15/30;C12N15/67;C12N15/74;C12N15/85;C12P19/34 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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