METHOD AND DEVICES FOR LASER INDUCED FLUORESCENCE ATTENUATION SPECTROSCOPY (LIFAS)

摘要

The Laser Induced Fluorescence Attenuation Spectroscopy method and apparatus include a source (311) adapted to emit modulated radiation that is directed at a sample volume in a sample (314) to produce return light from the sample, a first sensor (321), displaced by a first distance from the sample volume for monitoring the return light and generating a first signal indicative of the intensity of return light, a second sensor (322) displaced by a second distance from the sample volume for monitoring the return light and generating a second signal indicative of the intensity of return light, and a processor (323) adapted to process the first and second signals so as to determine the modulation of the sample. Methods and devices are well-suited for determining the wavelength-dependent attenuation of a sample and using the attenuation to restore the intrinsic laser induced fluorescence thereof, and can be used to determine the ischemic or hypoxic condition of biological tissue.