| 摘要 |
<p>Oligopeptide affinity ligands can be produced by generating a combinatorial library of short peptides (preferably from a limited set of amino acids) and then carrying out a double screening using first a component related to the target but to be distinguished from it (e.g. haemoglobin) and then the target (e.g. glycosylated haemoglobin). 'Virtual' combinatorial synthesis can be carried out by computer modelling. Ligands for targetting NbA1c include Boc-GAIIVL, Boc-VFMPLG and FPADP. The ligands can be used to produce biosensors and sensor systems, e.g. based on the binding of a ligand to its target causing changes in optical properties (e.g. refractive index, e.g. measured by surface plasmon resonance) or changes in electrochemical properties (e.g. when the ligand is bound to the surface of an electrode).</p> |
