发明名称 |
FUNCTIONAL ASSAY FOR TUMOR SUPPRESSOR GENES. |
摘要 |
<p>A method for determining whether an individual, i.e., a human, carries a mutation in a gene which encodes a transcriptional regulator (e.g., a tumor suppressor). The method includes the steps of obtaining a nucleic acid sample comprising cDNAs encoding both alleles of the gene from a cell sample from the individual; providing a nucleic acid construct comprising a binding site operably linked to DNA encoding a detectable protein such that transcription of the DNA encoding the detectable protein is regulated by interaction of the transcriptional regulator polypeptide with the binding site; transfecting the nucleic acid sample and the nucleic acid construct into cells with low levels of the transcriptional regulator polypeptide; and then determining the levels of the detectable protein in the transfected cells, this level being correlated with the presence of a mutation in at least one of the alleles of the gene.</p> |
申请公布号 |
EP0675967(A4) |
申请公布日期 |
1999.04.28 |
申请号 |
EP19930922429 |
申请日期 |
1993.09.28 |
申请人 |
THE GENERAL HOSPITAL CORPORATION;INSTITUT SUISSE DE RECHERCHES EXPERIMENTALES SUR LE CANCER |
发明人 |
FRIEND, STEPHEN H.;FREBOURG, THIERRY;ISHIOKA, CHIKASHI;IGGO, RICHARD |
分类号 |
G01N33/566;C12N5/10;C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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