METHOD OF DETERMINING THROMBOPHILIA CAUSED BY RESISTANCE OF V FACTOR TO ACTIVATED PROTEIN C

摘要

FIELD: clinical hematology. SUBSTANCE: to activate protein C, protein C activator is added to standard oxalate plasma and factor V-free plasma and then phospholipid membranes of platelets are added to mixture being studied. For diagnostics, one determines normalized ratio as ratio of index Rb for patient's plasma which is ratio of patient's plasma coagulation time, plasma containing no V factor and containing activated protein C to diluted patient's plasma coagulation time, to that of V factor-free plasma, and to index Rè for normal plasma. When normalized ratio is less than 0.8, results of test are regarded as positive. EFFECT: simplified procedure, improved specificity and reproducibility. 1 dwg, 3 ex