Non-antibiotic system for selection of recombinant mycobacteria

摘要

The present invention provides a Mycobacterium-E. coli shuttle vector containing sequences coding for a tribrid fusion containing the M. leprae 18kDa antigen sequence, a phoA protein antigen sequence and a non-mycobacterial, non-E.coli heterologous protein antigen sequence. Also provided is a method of producing protective immunity in an animal or human host in need of such treatment, comprising the step of administering to said animal or human host an effective dose of recombinant mycobacteria carrying the vector of the present invention.