摘要 |
Provided is a method of identifying agonists and antagonists of nuclear receptors that comprises measuring agonist-dependent fluorescence resonance energy transfer (FRET) between a fluorescently labeled nuclear receptor or ligand binding domain and fluorescently labeled CREB-binding protein (CBP), p300, other nuclear co-activator, or binding portion thereof. The method is simple, rapid, and inexpensive. Nuclear receptors and nuclear receptor co-activators labeled with fluorescent reagents for use in the above-described method are also provided.
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申请人 |
MERCK & CO., INC.;CUMMINGS, RICHARD, T.;HERMES, JEFFREY, D.;MOLLER, DAVID, E.;ZHOU, GAOCHAO |
发明人 |
CUMMINGS, RICHARD, T.;HERMES, JEFFREY, D.;MOLLER, DAVID, E.;ZHOU, GAOCHAO |