| 摘要 |
<p>A process for purification of plasmid DNA by separation on an insoluble matrix comprising: a) lysing cells containing said plasmid DNA to obtain a lysate; b) adding divalent alkaline earth metal ions to the lysate from a); c) removing precipitated compounds from the lysate from step b); d) contacting the lysate from step c) with an anion exchange matrix to obtain purified plasmid DNA. Preferably CaCl2 is added in step b) to provide the alkaline earth metal ions. Further the process comprises: e) contacting the purified plasmid DNA from step d) with an anion exchange matrix.</p> |
