Fremgangsmåde til karakterisering af et materiale af biologisk oprindelse under anvendelse af nær-infrarød spektroskopi

摘要

A method is provided for predicting a property of a matter of biological origin, such as biological fluid, containing water, where the biological matter may be approximated to contain two compartments where one compartment has a proportionally larger or smaller amount of water than the other compartment having the property of interest. The method involves establishing a training set in the near-infrared (NIR) region with independent quantification of the property of the matter using known techniques. The training set is mathematically analyzed according to a correlation developed by regression analysis after employment of a pre-processing technique. The result is a mathematical transformation equation which quantitatively relates spectral intensities at specific wavelengths to the property of interest. This transformation equation may be applied to unknown samples so as to predict their properties, thereby eliminating need for the reference method, except for validation and recalibration. The method provides rapid and accurate prediction of the property of the unknown sample, which may be the property of hematocrit or hemoglobin concentration in whole animal blood. The pre-processing technique may be the transformation of the spectra using a multiple derivative calculation, such as computing the second derivative of the several spectra of known and unknown samples.