A method to assess oxidative stress in vivo by measuring the amount of free, esterified and glucuronidated forms of isoprostanes (8EPGF2) in a biological sample which contains the isoprostanes is disclosed. The method further includes determining the amount of total isoprostanes present in the sample. This amount is compared with a control sample. The oxidative stress is determined through the comparison wherein the amount of isoprostanes increase in the sample isolated from an organism undergoing oxidative stress compared to the control. Alternatively the method of the present invention provides for only the measurement of the glucuronidated form wherein the amount of glucuronidated isoprostanes increase in the sample isolated from an organism undergoing oxidative stress compared to the control.
申请公布号
US5891622(A)
申请公布日期
1999.04.06
申请号
US19980028543
申请日期
1998.02.24
申请人
OXFORD BIOMEDICAL RESEARCH, INC.;VANDERBILT UNIVERSITY
发明人
MORROW, JASON D.;KIM, HYESOOK;ROBERTS, II, L. JACKSON;CALLEWAERT, DENIS M.