Improved method of production of pneumococcal surface proteins

摘要

The present invention provides improved methods for preparation of immunoligical or vaccine compositions comprising PspA-like polypeptides. Specifically, the present invention provides vaccine compositions wherein PspA or PspA-like polypeptide from Rx1 or other representatives of clade 2 or family 1 have been produced by recombinant methods. Recombinant constructs are provided which express a substantially homogeneous, truncated form of immunologically active PspA-like polypeptides in host cells transformed with such constructs.