| 摘要 |
PURPOSE:To enable rapid and accurate detection by adding 5-bromo-3-indolyl-beta- D-galactoside (Blugal), etc., to a culture medium for proliferating the coliform group. CONSTITUTION:A culture medium for detecting the coliform group is prepared by adding Blugal or 5-bromo-4-chloro-indolyl-3-D-galactoside (Xgal) in an amount of 10<-3> to 10<-4> M to a culture medium such as brain heart infusion agar culture medium (BHI) or tryptosoya agar culture medium (TSA). A coliform group such as the genus Escherichia, Citrobacter, Klebsiella, Enterobacter or Serratia, as necessary, is inoculated into the aforementioned culture medium and cultured at about 37 deg.C for about 18hr to carry out reaction and color the culture medium. Thereby, the presence of the coliform group is detected. |
