METHODS FOR PREPARING LIPIDS/POLYNUCLEOTIDE TRANSFECTION COMPLEXES

摘要

Methods are provided for the preparation of transfection complexes of polynucleotides and cationic lipids suitable for delivering polynucleotides to cells. In particular, polynucleotide/cationic lipid transfection complexes are prepared in the absence of detergent by methods in which polynucleotides and cationic lipids are solubilized in an aqueous solution in the presence of co-solvent, and the removal of co-solvent under conditions which allow polynucleotide and cationic lipid to assemble into transfection complexes. The process produces a substantially homogenous population of tranfection complexes and is scaleable.