发明名称 |
DIAGNOSTIC TEST FOR ALZHEIMER'S DISEASE |
摘要 |
A method for the diagnosis of Alzheimer's disease (AD) in a patient, comprising the steps of: (1) providing a sample of an appropriate body fluid from said patient; (2) detecting the presence of acetylcholinesterase (AChE) with an altered glycosylation pattern in said sample. It has been established that approximately 75-95 % of the AChE in the CSF of AD patients binds to Concanavalin (Con A) or wheat germ agglutinin (WGA) but with different specificity to each. Accordingly, in order to identify the glycosylation pattern of AChE in the sample, the binding to Con A is determined, then the binding to WGA is determined, and a ratio calculated. The ratio is characteristic of the glycosylation pattern. In an alternative embodiment of the invention a monoclonal antibody specific for AChE with an altered glycosylation pattern is used to detect its presence.
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申请公布号 |
CA2304949(A1) |
申请公布日期 |
1999.04.01 |
申请号 |
CA19982304949 |
申请日期 |
1998.09.24 |
申请人 |
THE UNIVERSITY OF MELBOURNE |
发明人 |
SMALL, DAVID HENRY;SAEZ-VALERO, JAVIER;SBERNA, GIAN |
分类号 |
G01N33/573;C12N9/16;C12N9/18;C12Q1/46;G01N33/68;(IPC1-7):C12Q1/46 |
主分类号 |
G01N33/573 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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