| 摘要 |
This invention provides a method, a reagent, and a kit for detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The invention also provides a reagent for use in detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The method of this invention comprises the steps of: (a) providing a solid phase containing at least a portion of a herpesvirus antigen; (b) introducing a patient specimen to the solid phase of step (a); (c) introducing at least one peptide capable of specifically binding IgM antibodies to herpesvirus to the solid phase of step (a); (d) allowing IgM antibodies to herpesvirus to specifically bind to the portion of antigen of herspesvirus and the peptide capable of specifically binding IgM antibodies to herpesvirus to the solid phase; and (e) determining the level of IgM antibodies to herspesvirus in the patient specimen.
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