METHODS FOR PRODUCING HETEROLOGOUS DISULFIDE BOND-CONTAINING POLYPEPTIDES IN BACTERIAL CELLS

摘要

Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments, the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.